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  |   271: 12387-12393, 1996. Karyological studies on various K-562 sublines have been classified into three groups (A,B,C) by Dimery, et al. ***p<0.0001, **p=0.0085, *p=0.013, mean significantly different from 1, one PubMed: 7769849. 59: 77-82, 1977. Please enable it to take advantage of the complete set of features! Cultures can be maintained by the addition or replacement of fresh medium. Non-Profit: Leukemia cell line; cell surface marker; flow cytometry. Leukemia 9: 858-862, 1995. K562 cells are colony-forming units-erythroid equivalent cells to erythroblasts, with the highest expression of GATA-binding factor 2 (GATA2), GATA1 and Friend of gata-1 (FOG1). 1 The molecule is a member of the scavenger receptor cystein-rich (SRCR) family class B 2,3 and has been identified as an endocytic receptor for hemoglobin-haptoglobin (Hb-Hp) complexes. Histone deacetylase inhibition improves dendritic cell differentiation of leukemic blasts with AML1-containing fusion proteins. 166: 546-550, 1981. $23.00, For-Profit: If you experience any issues with your products or services, please contact ATCC Customer Service at, We remain dedicated to protecting your data and experience throughout our platforms. $49.00 PubMed: 8621627, Grune T, et al. 2.2.5 Other Surface Markers of NK Cells CD2: CD2 is the surface protein of natural killer cells [2]. J. Biol. As THP-1 and U937 are very frequently used, we focus here on … Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. MSC surface markers (CD44, CD73, and CD90) can identify human MSC-derived extracellular vesicles by conventional flow cytometry Ramos T Sánchez-Abarca L Muntión S et al. Our Technical Support team may be able to help. J. Moreover, ERK1/2 signaling is not involved in cell endomitosis. 70: 5312-5328, 1996. Tusa I, Cheloni G, Poteti M, Silvano A, Tubita A, Lombardi Z, Gozzini A, Caporale R, Scappini B, Dello Sbarba P, Rovida E. Target Oncol. FAQ K562 cells constitutively express glycophorin-A, but not HLA molecules. Expression of FLT3 receptor and response to FLT3 ligand by leukemic cells. Leuk. Chem. K562 and THP1 cells were stained for 268 markers (including isotype and secondary antibody controls) following mock treatment or trypsin treatment. Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. PubMed: 3857109, Wu SQ, et al. $614.00 Blood 45: 321-334, 1975. Jurkat cells are pro-T to mature T-cells with the highest Neurogenic locus notch-1 homolog protein 1 (NOTCH1) expression. 271: 15504-15509, 1996. Chem. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cancer 23: 143-147, 1979. PubMed: 163658, Lozzio BB, Lozzio CB. The development of molecularly tailored therapeutic agents such as the BCR/ABL-active tyrosine kinase inhibitors (TKi) resulted in an excellent treatment option for chronic myeloid leukemia (CML) patients. 2016 Jan 12;14:2. doi: 10.1186/s12964-015-0124-8. bone marrow PubMed: 299761, Pross HF, et al. Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells. Home J. This site needs JavaScript to work properly. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. Natural cytotoxic reactivity of human lymphocytes against a myeloid cell line: characterization of effector cells. $2,249.10, ISO 17025 Molecules. Koeffler HP, Golde DW. Therefore it is likely that in the Privacy Policy PubMed: 7194480, Dimery IW, et al. Surface expression of 4-1BB ligand (4-1BBL) and membrane-bound IL-15 on K562/41BBL/mb15 and K562/41BBL/mb15/mb21 cells was assessed with 4-1BBL antibody (clone 282220) and IL-15 antibody (clone 34559; both R&D 271: 9546-9549, 1996. An important limitation in the field of NK research is attributable to the deficit of assays available for Background: Epub 2004 Jun 14. 1 They are important effectors of the innate immune system and contribute to the first line of defense against infections and malignancy. It is a NK cell trigger receptor with a size of 50-55 kDa and is also expressed in T cells. Unstable markers were also rarely seen. chronic myelogenous leukemia (CML), For-Profit: PubMed: 8557663, Tiffany HL, et al. The five markers indicated an inhibition of exosome secretion by K562 cells transfected with Rab11S25N. Hematol. Your account has been locked. Cancer Genet. Non-Profit: Studies conducted by Anderson, et al., on the surface membrane properties led to the conclusion that the K-562 was a human erythroleukemia line. Gu WY, Cao XS, Qiu GQ, Chen ZX, Sheng LX, Xie XB, He J, Cen JN, Shen HL. 2020 Aug 4;119(3):493-501. doi: 10.1016/j.bpj.2020.06.027. However, also be aware that K562 cell lines obtained from different sources may differ, for example in HLA class 2 molecule induction by IFN-gamma (see Ress SR, X96391 All Rights Reserved. 2017 Oct 17;18(10):2167. doi: 10.3390/ijms18102167. Their implementation in therapy will require standards for quality control, including appropriate selection criteria for functional analysis and efficacy determination. Relation of Natural Killer Cell Line NK-92-mediated Cytolysis (NK-92-lysis) With the Surface Markers of Major Histocompatibility Complex Class I Antigens, Adhesion Molecules, and … PubMed: 8663134 Jondal M, Pross H. Surface markers on human b and t lymphocytes K562 cells were maintained in complete media consisting of RPMI 1640 (Invitrogen) supplemented with 10% fetal bovine serum (FBS, Invitrogen). Results: Comparative analysis of cell surface proteins in chronic and acute leukemia cell lines. Synergistic interactions between overlapping binding sites for the serum response factor and ELK-1 proteins mediate both basal enhancement and phorbol ester responsiveness of primate cytomegalovirus. Each point represents the measurement of a surface protein after mock or  /  Cancer 15: 596-605, 1975.   |   Keywords: PubMed: 806545, West WH, et al. Homo sapiens, human Int. These permits may be required for shipping this product: For-Profit: PubMed: 69036, Lozzio CB, Lozzio BB. Elongation Index as a Sensitive Measure of Cell Deformation in High-Throughput Microfluidic Systems. $2,646.00   |   PubMed: 8764042, Kolanus W, et al. 1: 51-63, 1981. Properties and usefulness of the original K-562 human myelogenous leukemia cell line. Degradation of oxidized proteins in K562 human hematopoietic cells by proteasome. NK-92 cells were cultured in GH T551 H3 medium (Takara Biomedical Technology (Beijing) Co., Ltd) and 500 IU/mL IL-2 (Shandong Quangang Pharmaceutical Co., Ltd). Exp. $1,246.00 Are you sure? Homo sapiens cell-line K562 sodium/hydrogen exchanger isoform 1 mRNA, complete cds. USA.gov. $614.00, For-Profit: Cancer Inst.  |  Two distinct upstream regulatory domains containing multicopy cellular transcription factor binding sites provide basal repression and inducible enhancer characteristics to the immediate-early IES (US3) promoter from human cytomegalovirus. Abstract The K562 cell line derived from a CML patient in blast crisis was examined for properties of B and T lymphocytes and cell lines. Biol. Lee SJ(1), Kim KH, Park JS, Jung JW, Kim YH, Kim SK, Kim WS, Goh HG, Kim SH, Yoo JS, Kim DW, Kim KP. J. Biol. The PI3K pathway exerts dual regulatory effects on K562 cell differentiation: it is intimately connected with ERK1/2 cascade toand it K-562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognizable progenitors of the erythrocytic, granulocytic and monocytic series. Internal initiation of translation directed by the 5'-untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from cap-dependent to internal initiation. Specificity of natural cytotoxic reactivity of normal human lymphocytes against a myeloid leukemia cell line.   |   NLM PubMed: 95026, Andersson LC, et al. Soc. Start new cultures at 1 x 10, Ethical Standards for Obtaining Human Materials, Iscove's Modified Dulbecco's Medium (IMDM) (ATCC, Nucleic Acids, Proteins and Cell Extracts. Don't see what you are looking for? PubMed: 8647842, Chan YJ, et al. 271: 15504-15509, 1996. NB4 and MV-4-11 express high levels of CCAAT/enhancer-binding protein-alpha (CEBPα) and differentiate from myeloblasts to pro-monocytes and myeloblasts, respectively. Enter your user name and click Submit. $938.00 Contact Us Variation amongst K562 cell cultures. Degradation of oxidized proteins in K562 human hematopoietic cells by proteasome. $1,246.00, For-Profit: Conversely, we have observed an increase in vesicle release in Rab11wt overexpressing cells. Differentiation toward mega-karyocytes can be monitored by a reduction of growth potential (22), a reduction of expression of erythroid surface markers (15), a reduction of c-myc expression (18, 23 The strain obtained by the ATCC most closely resembles the B population. Quality Accreditations. Exosomes released by imatinib‑resistant K562 cells contain specific membrane markers, IFITM3, CD146 and CD36 and increase the survival of imatinib‑sensitive cells in … Do Jurkat E6.1 cells express CD4 surface marker? The stemline chromosome number is triploid with the 2S component occurring at 4.2%. K562 cells can be induced to express markers associated with megakaryocytic differentiation when incubated with PMA . Ferritin Heavy Subunit Silencing Blocks the Erythroid Commitment of K562 Cells via miR-150 up-Regulation and GATA-1 Repression Int J Mol Sci . 11: 601-610, 1983. ATCC stands ready to support our customers’ needs during the coronavirus pandemic. NCI CPTC Antibody Characterization Program. K562--a human erythroleukemic cell line. K562 cells are of the erythroleukemia type, and the cell line is derived from a 53-year-old female chronic myelogenous leukemia patient in blast crisis. NIH Epub 2012 Dec 14. HHS   |   The quantitation of natural killer cell activity. Also, it expresses mature monocyte markers that cannot be found on the THP-1 and U937 cell lines, such as M42, LeuM3, 63D3, Mo2 and UCHMI. IX. Cell 86: 233-242, 1996. J. Biol. Zdechlik AC, He Y, Aird EJ, Gordon WR, Schmidt D. Bioconjug Chem. J. Natl. CD163 is a cell-surface glycoprotein receptor that is highly expressed on most subsets of resident tissue macrophages. Extensive amplification of bcr/abl fusion genes clustered on three marker chromosomes in human leukemic cell line K-562. PubMed: 8706128, Gan W, Rhoads RE. The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. Flow cytometric analysis of DNA content in intact cells treated with Hoechst 0 1 These include the human killer 2020 Feb 11;25(4):765. doi: 10.3390/molecules25040765. Enhanced expression of the eosinophil-derived neurotoin ribonuclease (RNS2) gene requires interaction between the promoter and intron. Human myeloid leukemia cell lines: a review. In addition to the markers which are commonly used to identify mouse and human NK cells by flow cytometry, NK cells also express multiple cell surface receptors that regulate their activation. Chem. Spontaneous non-specific dicentrics occurred, but rarely. ISO 9001 Res. Gene. Non-Profit: Proc. They can be induced to express HLA class 1 molecules by IFN-gamma. MSC surface markers (CD44, CD73, and CD90) can identify human MSC-derived extracellular vesicles by conventional flow cytometry Teresa L. Ramos1,2,3,4†, Luis … A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. 2004 Sep;76(3):623-33. doi: 10.1189/jlb.1103581. Epub 2019 Dec 20. J. Biol. Effect of the R569W missense mutation on the biosynthesis of myeloperoxidase. [Immunotherapy for leukemia cells by using cytotoxic T lymphocyte specifically against WT1-derived peptide: an experimental study in vitro]. Differentiation states of Kasumi-1 and MOLM-13 cells are colony-forming units-granulocyte/macrophage equivalent cells to myeloblasts with comparatively high Growth factor independent-1(GFI1) and Transcription factor PU.1 (PU.1) expression, respectively. Chem. By switching countries your current shopping cart will be cleared. Nucleotide (GenBank) : Non-Profit: Nucleotide (GenBank) : AF146430 Specifically, a lack of CD107a surface expression after incubation with K562 target cells is consistent with syntaxin-11, mammalian uncoordinated 13.4, syntaxin-binding protein 2, or Rab27A protein defects.54, 55, 56, 57 $320.00 © 2020 ATCC. Exp. The cells are non-adherent and rounded, are positive for the bcr:abl fusion gene, and bear some proteomic resemblance to … Elton TS, Selemon H, Elton SM, Parinandi NL. K562 cells are colony-forming units-erythroid equivalent cells to erythroblasts, with the highest expression of GATA-binding factor 2 (GATA2), GATA1 and Friend of gata-1 (FOG1). The X was disomic, and N9 was nullisomic. K562—A human erythroleukemic cell line K562—A human erythroleukemic cell line Andersson, Leif C.; Nilsson, Kenneth; Gahmberg, Carl G. 1979-02-15 00:00:00 W e have studied the surface membrane properties of the human leukemic cell line K562 which previously has been reported t o represent an early stage of granulocyte maturation. 17: 55-60, 1985. Characterization of adult human marrow hematopoietic progenitors highly enriched by two-color cell sorting with My10 and major histocompatibility class II monoclonal antibodies. 271: 623-626, 1996. Careers Non-Profit: PubMed: 6576909, Chan YJ, et al. K562 Cell Line Surface Markers Ian Titley Ian.Titley at icr.ac.uk Fri Dec 15 04:41:03 EST 2006 Previous message: Guava EasyCyte Plus Next message: better filters to distinguish GFP from autofluorescence? PubMed: 367973, Lozzio BB, et al. MSC surface markers (CD44, CD73, and CD90) can identify human MSC-derived extracellular vesicles by conventional flow cytometry Cell Commun Signal . Characterization of Cell Surface Proteins. Leukemia cell lines are utilized as tools for molecular analysis. Med.  |  (MEL), and K562 cells (20, 21). (E) Flow cytometry measurements of the indicated cell surface markers in K562 cells after indisulam treatment. This cell line is suitable as a transfection host. COVID-19 is an emerging, rapidly evolving situation. 70: 8590-8605, 1996. The cell population has been characterized as highly undifferentiated and of the granulocytic series. Non-Profit: 30-2005. Conclusion: Author information: (1)Department of Molecular Biotechnology, Institute of Biomedical Science and Technology, Konkuk University, Seoul 143-701, Republic of Korea. $49.00.  |  K562 and K562-ABCB1 cells were treated continuously either with 0.5 µM or 25 µM imatinib in the presence or absence of 10 µM PSC833 as indicated. J. Virol. The cell line described here thus displays characteristics of activated NK-cells and Jurkat cells are pro-T to mature T-cells with the highest Neurogenic locus notch-1 homolog protein 1 (NOTCH1) expression. Clipboard, Search History, and several other advanced features are temporarily unavailable. PubMed: 6996765, Ortaldo JR, et al. Grune T, et al. Non-Profit: NK-92 ® is an interleukin-2 (IL-2) dependent Natural Killer Cell line derived from peripheral blood mononuclear cells from a 50 year old Caucasian male with rapidly progressive non-Hodgkin's lymphoma. 2020 Apr 15;31(4):1093-1106. doi: 10.1021/acs.bioconjchem.9b00790. Question 1 answer Asked 27th Feb, 2015 Ursula Chong I tried staining the Jurkat E6.1 cells with CD4-PE but there were little to … Tissue: Natural killer (NK) cells are a subset of lymphocytes that play a central role in the innate immune response to tumors and infections. Characteristics of six human leukemia cell lines -Kasumi-1, NB-4, MOLM-13, MV-4-11, K562, and Jurkat cells-were investigated using multiple color analysis of surface antigen expression and comparative analysis of gene expression. Blood 56: 344-350, 1980. The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises. Modal karyotype of human leukemia cell line, K562 (ATCC CCL 243). PubMed: 8970984, Nauseel WM, et al. Int.  /  2013 Dec 10;532(1):1-12. doi: 10.1016/j.gene.2012.12.009. Next, the detection limits of this kit for exosomes purified from K562 cells (of human leukemia origin) and COLO201 cells were determined to be 49.9 pg and 10.9 pg, … H.sapiens mRNA for alternatively spliced isoform of CLC-6 chloride channel. NK-92 cells kill both K562 and Daudi cells very effectively in a 4 h51-chromium release assay (84 and 86% respectively, at an E:T cell ratio of 5:1). Fifteen markers (M1 and M(15)) occurred in nearly all S metaphases. Chem. Immunol. Cells exposed to … Biophys J. $320.00, For-Profit: When bound to ligands on tumor cells, CD2 K562-S and K562-R cell surface proteins were radiolabeled following the methods described by Markwell and Pox (17). J. Clin. A multipotential leukemia cell line (K-562) of human origin. We have updated our. Spontaneous human lymphocyte-mediated cytotoxicity against tumor target cells. Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome. megakaryocyte surface markers. 2005 Dec 28;85(49):3475-80. Human natural killer (NK) cells comprise approximately 10% of peripheral blood (PB) lymphocytes and are characterized phenotypically by the expression of CD56 and the lack of CD3 cell surface antigens. J Leukoc Biol. In Vitro Comparison of the Effects of Imatinib and Ponatinib on Chronic Myeloid Leukemia Progenitor/Stem Cell Features. The red blood cell (RBC) is the final stage of a journey that starts in the erythropoietic organs [ 1 ] with the maturation of hematopoietic stem cells. Disease:   |   $797.30, For-Profit: Alvieri F, Mamani JB, Nucci MP, Oliveira FA, Filgueiras IS, Rego GNA, de Barboza MF, da Silva HR, Gamarra LF. Epub 2020 Jul 7. VI. Our study gives a useful guideline of standards for appropriate usage of leukemia cell lines for examining novel targets in vitro. Zhonghua Yi Xue Za Zhi. K562 lacks the B markers … Mean and standard deviation are shown. To further explore the role of p53 along different lineages of differentiation, K562/ptsp53 clones and control clones were incubated with or without PMA at different concentrations at the permissive temperature. surface markers such as CD98 revealed that fixation with 2-4% paraformaldhyde followed by permeabiliza-tion with 0.1% saponin give the best result. Organism: Methods of Granulocyte Isolation from Human Blood and Labeling with Multimodal Superparamagnetic Iron Oxide Nanoparticles. Despite the fact that K562 erythroleukemia cell lines are of neoplastic origin, this cell line offers a versatile model system to research the regulatory mechanisms underlying erythropoiesis. All cells were cultured at 37 °C 5% CO 2. Cytogenet. Occurrence of the Philadelphia chromosome, however, was of much lower frequency; none detected in 15 metaphases examined. The effect of inducers on sublines derived from the original K-562 cell line have been reviewed by Koeffler and Golde. 118: 355-361, 1977. 2020 Oct;15(5):659-671. doi: 10.1007/s11523-020-00741-x. 3: 363-370, 1979. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. J. Immunol. Copyright© 2014 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved. J. Biol. Materials and methods: Regulation of the MIR155 host gene in physiological and pathological processes. Cytotoxicity against cell lines as a functional marker for lymphocyte subpopulations. PubMed: 8663134, Jondal M, Pross H. Surface markers on human b and t lymphocytes. J. Virol. PubMed: 7334070, Chen TR. Lu L, Walker D, Broxmeyer HE, Hoffman R, Hu W, Walker E. Moldenhauer A, Frank RC, Pinilla-Ibarz J, Holland G, Boccuni P, Scheinberg DA, Salama A, Seeger K, Moore MA, Nimer SD. An email will be sent to you with instructions. $23.00 Programmable Assembly of Adeno-Associated Virus-Antibody Composites for Receptor-Mediated Gene Delivery.

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